Grouping Molecules into Subpopulations

About grouping

Dividing molecules into subgroups is an efficient way of characterizing sample heterogeneity, i.e. the diversity of different molecules, as well as to quickly plot sub-populations of FRET-pairs. Grouping must be activated in the toolbar of the FRET-pair window:

Once grouping is activated the groups are listed in the list box to the left and FRET-pairs are named according the group they belong to. Initially, all molecules are put in the default group 'A'.

Create new group

 

Go to 'Grouping->Create new group'.
Specify the name of the new group and press OK.
The new group is added to the group listbox and molecules put in the group are renamed in the FRET-pairs list box.

Put molecules in a group

Select the molecule(s) in the FRET-pairs list box and the target group(s) in the group list box. Then press Ctrl+G (on windows), or navigate to 'Grouping->Put selected molecules in selected group'.
The molecule is now added to the selected group.
If you want the molecule to belong to more than one group, untick the menu item 'Grouping->Remove from previous groups...' before grouping the molecule.

Sort molecules according to group

Select 'Sort->According to group'. This rearranges molecules in the list box so they are listed according to the group to which they belong, in the order defined in the group list box.
To specify the group-order appearance, e.g. when molecules belong to more than one group, select 'Grouping->Sort groups'.
In the dialog, rearrange group order using the two arrows. Then press OK.
The group order is now changed.

Modify group properties

To change group names or colors, navigate to 'Grouping->Rename groups' and/or 'Grouping->Color groups'.

Delete group

To delete a group (but not the molecules in it) go to 'Grouping->Delete group'. Select the group in the dialog and press OK. If some molecules in the deleted group are left without a group, a dialog will prompt for a target group of these molecules.