Filtering traces

iSMS provides the possibility to plot and inspect filtered time traces. Filters include:

  • Zero-phase infinite impulse response (IIR) lowpass filter: For more info see MATLAB's documentation on filtfilt and designfilt.
  • 1-D median filter: For more info see MATLAB's documentation on medfilt1 here.

Note that the filtering tool is only for visual inspection and testing purposes. It is not possible to apply filtered traces for further analysis and plotting in iSMS.

In the FRET-pairs trace window go to Plot->Filter traces
In the new window, select the filter type and settings. This figure shows an example of the zero-phase infinite impulse response (IIR) lowpass filter. Specify filter order and half power frequency.
Specify the window size of the median filter in frames.